Case analysis of incompatibility in blood crossmatching induced by anti-Dia and anti-E antibodies / 国际检验医学杂志

ABSTRACT

Objective To investigate the reason of anti-Dia combined with anti-E antibodies inducing incompatibility in blood crossmatching.Methods Retrospectively analyse a case of patient treated in the hospital,whose blood crossmatching failed for sev-eral times.Through blood typing,direct Coombs test,irregular antibody screening,adsorption and elution experiments to determine the patient′s blood type,possible irregular antibodies,cell spectral response pattern.Results Forward blood typinganti-A antibody (-),anti-B antibodies(-);Reverse blood typingAc (++++),Bc(++++),RBC(-),Oc(-);Rh blood groupinganti-c an-tibody(-),anti-C antibody (+ + + +),anti-D antibody (+ + + +),anti-e antibody (+ + + +),anti-E antibody (-),direct Coombs test(-).Patient′s serum contained anti-E and anti-c antibodies.Conclusion Irregular antibodies in the serum of patients is an important cause of crossmatch discord.