Screening and primary analysis of microRNAs expression profile in human breast cancer BT474 cells with different lever of HER-2 / 临床与实验病理学杂志

ABSTRACT

Purpose To detect the expression profile of microRNAs ( miRNAs) in human breast cancer cells BT474 and BT474/ siR-NA HER-2, and to screen out miRNA expression profile related to HER-2. Methods RNA interference was used to inhibit HER-2 expression in human breast cancer line BT474. The expression profile of miRNAs was detected by miRNA microarray technique. Re-sults According to discrepancy power >2. 0 and P value<0. 05, there were 338 miRNAs related to HER-2, including 110 up-regu-lated and 228 down-regulated miRNAs. Conclusion miRNA microarrays can detect the differentially expressed miRNAs in human breast cancer BT474 cells with different level of HER-2. The miRNAs related to HER-2 which play a role in breast cancer should be found after further analysis.