Hinokitiol induces clear cell renal cancer 786-O cell apoptosis via autophagy induction / 中国肿瘤临床
Chinese Journal of Clinical Oncology
;
(24): 43-46, 2015.
Artigo
em Chinês
| WPRIM
| ID: wpr-462593
ABSTRACT
Objective:
To investigate the effects of hinokitiol on the proliferative inhibition and apoptosis induction in human clear cell renal cancer 786-O cells.Methods:
CCK-8 assays were performed to analyze the effects of hinokitiol on the proliferation of 786-O cells. The apoptosis rate was determined by flow cytometry. EGFP-LC3 microscopy assays were performed to assess the autoph-agy flux. Cleaved Caspase-3, LC3, and P62 were detected by Western blot.Results:
Hinokitiol could inhibit the proliferation of the 786-O cells and could induce cell apoptosis via Caspase pathway. Hinokitiol induced the autophagy of 786-O cells, increased LC3 ex-pression, and downregulated P62 expression.Conclusion:
Hinokitiol can inhibit the proliferation of 786-O cells and can induce cell apoptosis via autophagy induction.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Idioma:
Chinês
Revista:
Chinese Journal of Clinical Oncology
Ano de publicação:
2015
Tipo de documento:
Artigo
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