Enrichment of lung cancer stem cells and expression of related markers / 中国组织工程研究

ABSTRACT

BACKGROUND:Lung cancer stem cel s are tightly related to the treatment and prognosis of lung cancer. We can provide more references for clinical diagnosis and treatment of lung cancer through the study on the tumorigenicity and surface markers of lung cancer stem cel s. OBJECTIVE:To explore the enrichment methods for lung cancer stem cel s and cel ular tumorigenicity. METHODS:Lung cancer stem cel s were induced in serum-free culture medium containing epidermal growth factor, insulin-like growth factor 1, and basic fibroblast growth factor. Then, the expressions of related surface markers were detected using immunofluorescence method. After that, mice were implanted subcutaneous with lung cancer stem cel spheres to understand the tumorigenicity of lung cancer stem cel s. RESULTS AND CONCLUSION:Lung cancer stem cel s under serum-free induction and culture were changed to sphere-forming cel s, and the immunofluorescence detection showed that over 80%of sphere-forming cel s were positive for CCSP, SP-C and OCT4. After transplantation of sphere-forming cel s, the mice showed a high tumorigenicity. These findings indicate that sphere-forming cel s are formed after serum-free suspension culture of lung cancer stem cel s, which have a higher tumorigenicity.