Study on HPLC Fingerprint of Total Flavonoids from Pollen ofBrassica campestrisL / 世界科学技术-中医药现代化
World Science and Technology-Modernization of Traditional Chinese Medicine
; (12): 591-595, 2015.
Article
em Zh
| WPRIM
| ID: wpr-464809
Biblioteca responsável:
WPRO
ABSTRACT
In this article, HPLC fingerprint analysis method for total flavonoids from pollen of Brassica campestris L.was established., The HPLC fingerprint was performed on Waters C18 column (250 mm × 4.6 mm, 5 μm), eluted gradiently with the mixture of acetonitrile and 0.4% phosphoric acid aqueous solution at a flow rate of 0.8 mL·min-1. The column temperature was 40℃. The detection wavelength was 320 nm. The HPLC standard fingerprint of total flavonoids from pollen of Brassica campestrisL. was established, and 16 common peaks were calibrated. The method was simple, stable, and reproducible. It could be applied for quality control of total flavonoids from pollen of Brassica campestris L.
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Índice:
WPRIM
Idioma:
Zh
Revista:
World Science and Technology-Modernization of Traditional Chinese Medicine
Ano de publicação:
2015
Tipo de documento:
Article