Optimization of Extraction Process of Effective Constituents from Rosae Laevigatae Frustus by Box-Behnken Response Surface Method / 中国中医药信息杂志

ABSTRACT

Objective To screen extraction process for Rosae Laevigatae Fructus.Methods With the content of total saponins and total phenolic acids from Rosae Laevigatae Fructus as the evaluation indexes, ethanol concentration, extraction time, solid-liquid ratio and extraction times as investigation factors, the test data were analyzed by Design-Expert8.0.5 software, and a multiple quadratic regression equation was set up to screen extraction process parameters of Rosae Laevigatae Fructus.Results The best extraction process parameters of Rosae Laevigatae Fructus were as followsethanol concentration was 62.79%;solid-liquid ratio was 13.121;the extraction time was 137.93 min;extraction times were 1.85 times. Considering the convenience for actual operation and the practicability of industrial production, the extraction process parameters should be ethanol concentration of 60%, revised solid-liquid ratio of 131, 2 hours' extracting time, and 2 extraction times.Conclusion Final selected parameters provide the basis for the extraction process of Rosae Laevigatae Fructus.