Genotyping analysis of epidemic strains ofMycoplasma pneumoniae in Suzhou area / 临床儿科杂志
Journal of Clinical Pediatrics
; (12): 338-341, 2015.
Article
em Zh
| WPRIM
| ID: wpr-465770
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WPRO
ABSTRACT
ObjectiveTo analyze the genotype and variation ofMycoplasma pneumonia (MP) strains isolated from chil-dren with MP infection in Soochow area.MethodsThe nasopharyngeal secretions from hospitalized children with MP infection were collected during January 2012 and December 2013. The nested-multiplex PCR based on MPP1 gene was performed to detect the subtype ofMP gene.ResultsIn 313 samples, 304 (97.12 %) samples were classiifed as P1-I type and 8 (2.56%) sam-ples were classiifed as P1-II type and one (0.32%) was V2 variant. Gene sequencing results were consistent with nested-multiple PCR results.ConclusionsNested-multiplex PCR is a reliable method for genotyping of MPP1 gene. During the study period, P1-I type was the common genotype and only one case of V2 variant was found.
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WPRIM
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Zh
Revista:
Journal of Clinical Pediatrics
Ano de publicação:
2015
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Article