Physiologic Role of K+ Channels in Relaxation of Rabbit Corpus Cavernosal Tissue / 대한남성과학회지
Korean Journal of Andrology
;
: 137-142, 2002.
Artigo
em Coreano
| WPRIM
| ID: wpr-46580
ABSTRACT
PURPOSE:
Ion channels play key roles in determining smooth muscle tone by setting the membrane potential and allowing Ca2+ influx. Potassium channels may be important in modulating corporal smooth muscle tone. In this study, we investigated the effects of potassium channels in the rabbit corpus cavernosal smooth muscle by blocking them with various agents. MATERIALS ANDMETHODS:
Strips of rabbit corpus cavernosum were prepared for mounting and isometric tension measurement in an organ bath. On cavernosal strips contracted with phenylephrine (PHE), sodium nitroprusside (SNP) was applied in increasing concentrations from 10(-7)M to 10(-4)M, causing dose-dependent relaxation. The effects of various potassium channel blockers on SNP-induced relaxation were then evaluated by measuring the tension of the cavernosal strips. The potassium channel blockers used were tetraethyl ammonium (TEA), charybdotoxin, gliben clamide, and apamin.RESULTS:
The relaxation responses to SNP of the corporal preparations contracted in response to PHE were significantly attenuated by TEA (10(-2)M) and charybdotoxin (10(-7)M), with no significant difference observed between the two drugs. The SNP-induced relaxation responses were not significantly attenuated by glibenclamide (10(-5)M) or apamin (10(-5)M).CONCLUSIONS:
These results suggest that maxi-K+ channels play an important role in corpus cavernosal relaxation. The KATP channel and small-conductance KCa channel are thought to be unrelated to corpus cavernosal smooth muscle relaxation.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Apamina
/
Fenilefrina
/
Relaxamento
/
Chá
/
Banhos
/
Nitroprussiato
/
Canais de Potássio
/
Glibureto
/
Charibdotoxina
/
Bloqueadores dos Canais de Potássio
Idioma:
Coreano
Revista:
Korean Journal of Andrology
Ano de publicação:
2002
Tipo de documento:
Artigo
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