The relationship between matrix Gla protein gene polymorphism, serum osteocalcin levels and bone mineral density in postmenopausal Korean women / 대한산부인과학회지

ABSTRACT

OBJECTIVE: To investigate the relationship between the matrix Gla protein (MGP) gene polymorphism, serum osteocalcin levels and bone mineral density (BMD) in postmenopausal Korean women. METHODS: The MGP gene cytosine-adenine (CA) polymorphism was analyzed by polyacrylamide-urea gel eletrophoresis, genescan and DNA sequencing in 267 postmenopausal Korean women. Serum osteocalcin, bone alkaline phosphatase (BAP), and CrossLaps (CTX) were measured by immunoassay, and enzyme linked immunosorbent assay respectively. BMD at the lumbar spine and proximal femur was determined by dual energy X-ray absorptiometry. RESULTS: Six MGP alleles were observed with product sizes ranging between 204-214 bp, and their distributions were as follows 210 bp 57.5%, 221 bp 27.9%, 206 bp 13.3% etc. There were no significant differences in BMD of the lumbar spine and proximal femur across the MGP genotypes. No significant differences in the distribution of MGP genotypes among normal, osteopenic, and osteoporotic postmenopausal women were observed. Serum osteocalcin level was significantly higher in women who did not 210 bp MGP (CA) allele than in women carrying at least one copy of this allele, while no significant differences in serum BAP and CTX levels were noted among MGP genotypes. CONCLUSION: The MGP gene (CA) polymorphism does not affect BMD but serum osteocalcin levels in postmenopausal Korean women.