Influence factors of microdialysis linear probe recovery for in vivo and in vitro cyclophosphamide calibration by HPLC-MS/MS / 国际药学研究杂志
Journal of International Pharmaceutical Research
;
(6): 362-364, 2015.
Artigo
em Chinês
| WPRIM
| ID: wpr-467811
ABSTRACT
Objective To establish an optimal linear probe recovery correction method for in vivo and in vitro cyclophosphamide. Methods with ringer solution as the perfusion, retrodialysis gain and loss method were used to calibrate the linear probe. The impact of different perfusion flow rates on microdialysis relative recovery for cyclophosphamide was investigated by HPLC-MS/MS. Results When the perfusion was 1.0μl/min, in vitro recovery results were in the range of (24.11±1.75)%to (50.93±0.91)%for the gain method, and(26.19±0.76)% to(53.31±1.23)% for the loss method;recovery results in vivo were(26.19±0.76)%. Conclusion Cyclophosphamide is suitable for microdialysis sampling. This experiment calibration method is simple and effective.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Tipo de estudo:
Estudo prognóstico
Idioma:
Chinês
Revista:
Journal of International Pharmaceutical Research
Ano de publicação:
2015
Tipo de documento:
Artigo
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