Study of pleural isolated Pseudomonas aeruginosas of the resistance mechanisms to carbapenems / 国际检验医学杂志

ABSTRACT

Objective To analyze the antibiotic phenotype of Pseudomonas aeruginosas that were isolated from pleural samples in our hosptial and test relative genes that may cause antibiotic resistance in order to provide information for clinical treatment . Methods BioMerieux Bact/ALERT 3D 120 blood culture system was used to culture pleural samples .Simens MicroScan Walk‐Away 96 was employed for bacterial identification and susceptibility test .Imipenem‐EDTA double disk method was used for screen‐ing MBL‐producing isolates .PCR was used for testing OprD ,VIM and IMP .Results 14 of 38 Pseudomonas aeruginosas isolates showed resistance to Imipenem and 2 were with multi‐resistance .4 isolates produced MBL .Only two were found lack of OprD gene among the 14 isolates ,and IMP and VIM genes were detected in 2 isolates .Conclusion The Pseudomonas aeruginosas from pleural infection show relative higher resistant rate to Imipenem ,and the exsiting mechanisms of Imipenem resistance included MBL (VIM and IM P genes) and lack of OprD .