Studies on Content Changes of Ginseng and Radix Puerariae before and after Compatibility / 世界科学技术-中医药现代化

ABSTRACT

This study was aimed to analyze differences of chemical compounds of Ginseng and Radix Puerariae be-fore and after compatibility using HPLC. Hypersil ODS column (4.6 mm × 250 mm, 5μm) was adopted. The mobile phase was acetonitrile-water for gradient elution. The detection wavelength was set at 203 nm. The column tem-perature was 25℃. The flow rate was 1 mL/min. The results showed that through the study of all main peaks in the finger print spectra, there was no obvious influence on extract before and after compatibility of Ginseng and Radix Puerariae. It was concluded that there were no obvious chemical changes of Ginseng and Radix Puerariae before and after compatibility. The synergistic mechanism of compatibility might mainly come from the interaction between the pharmacological actions and the absorption or the metabolism of effective constituents of the medicinal plants.