Using the technique of amplyfication of nucleic acide arrangement (NASBA) RNA to detect the bacilli of M.leprae in leprosy patient after treatment

ABSTRACT

The detection of structural ARN in ribosom-16sr RNA existing in living M.leprae has the value of valuating the efficacy of the treatment and make prognosis of the recurrence . NASBA is performed on 91 samples of biopsy of ear tissue of 2 groups of patients immediately after the treatment of the group I and 5 years after the treatment of the group II. In group I, the positive rate of NASBA is 1/29 and in group II is 3/62. All patients with positive NASBA are in the group of numerous bacilli (MB). In finishing the treatment, there is only 1 patient of MB group who has positive NASBA. Among 60 patients who finishes multichimiotherapy, after 5 years there are 3 patients with positive NASBA