Detection of meningeal bacteris in cerebrospinal fluid by multiplex PCR
Journal Ho Chi Minh Medical
;
: 58-61, 2004.
Artigo
em Vietnamita
| WPRIM
| ID: wpr-4744
ABSTRACT
Semi-nested multiplex PCR was used for rapid detection of Haemophilus influenzae, Niesseria meningitidis bacterials and streptococci in cerebrospinal fluid of suspect purulent meningitis patients. The method based on bacterial 16SrRNA gene amplification to be in all bacteria. With high specificity and sensitivity, the method had a detection limit of 10o CFU/ml on S.pneumoniae culture. Tested on 49 cerebrospinal fluid samples, the method detected 14/15 infected samples, in which 3 cases were infected with bacteria other than those investigated
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Bactérias
/
Líquido Cefalorraquidiano
/
Reação em Cadeia da Polimerase
/
Diagnóstico
Tipo de estudo:
Estudo diagnóstico
Idioma:
Vietnamita
Revista:
Journal Ho Chi Minh Medical
Ano de publicação:
2004
Tipo de documento:
Artigo
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