Determination of Allantoin in Rhizoma Dioscoreae by HILIC-HPLC-ELSD / 中国药师

ABSTRACT

Objective:To establish an HILIC-HPLC-ELSD method for the determination of allantoin in Rhizoma Dioscoreae. Methods:The separation was performed on a Waters XBridge HILIC column(150 mm × 4. 6 mm,5μm),the mobile phase was ace-tonitrile-water(8515)with the flow rate of 0. 8 ml·min-1 ,and the nebulizer pressure was 40psi,the drifting tube temperature was 80℃ and the nebulizer temperature was 50℃. Results:The linearity of allantoin was good within the range of 1. 5-7. 5 μg( r =0. 998 1). The regress equation was Y=1. 67X+2. 35,the detection limit was 25 ng,and the average recovery of allantoin in Rhizoma Dioscoreae was 99. 9% with RSD of 0. 72%(n=9). Conclusion:The method is simple and reliable,which can be used in the deter-mination of allantoin in Rhizoma Dioscoreae.