The Effects of Ischemic Acute Kidney Injury on the Morphology of Hippocampal CA1 Neurons in Rats / 天津医药

ABSTRACT

Objective To observe morphological changes of hippocampal CA1 neurons induced by ischemic acute kidney injury (IAKI) in rats.Methods After successful preparation of rat model of IAKI, the morphological changes of hip-pocampal CA1 neurons were observed by light microscope and electron microscope. The expressions of polyADP-ribose polymerases (PARP)-1 and caspase-3 in hippocampal CA1 neurons were detected by immunohistochemical (IHC) staining and Western blot assay.Results Pyknotic neurons in hippocampal CA1 area were found after 60 min ischemia and 24 h re-perfusion in kidney. Results of electron microscope showed that swollen mitochondria and dilated endoplasmic reticule in cy-toplasm and shrinkage nucleus with no pyknotic chromatin in the pyknotic neurons. IHC staining showed the negative cas-pase-3 staining and positive PARP-1 staining in pyknotic neurons. Conclusion The pyknotic neurons induced by IAKI might be mediated by PARP-1.