Effects of Cyclic Tensile Strain on Proliferation of Human Osteoarthritis Chondrocytes / 天津医药

ABSTRACT

Objective To explore the effects of cyclic tensile strain on the proliferation of cultured human osteoar-thritis chondrocytes. Methods The degenerated human cartilage came from a 66-year-old patient with osteoarthritis. The degree of degeneration was assessed by pathological diagnosis. Under the sterile condition, degenerated chondrocytes were cultured by enzymatic digestion. The toluidine blue staining, safranin O staining, and immunofluorescence staining of colla-genⅡwere used to identify the cultured cells. The third generation cells were seeded on the silicone rubber membrane carri-er, using the EF3200 mechanical tester equipped with BioDynamic bioreactor system, imposed a frequency of 0.25 Hz, stain 0, 5%, 10%and 15%load for 3 h. The proliferation activity was detected by flow cytometry. Results The proliferative in-dex (PI) increased with the magnitude value of cyclic tensile strain. The most significant increase of proliferation index was found in 10%group. Conclusion The proliferation of human osteoarthritis chondrocytes increases under some stress.