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Alpha-Melanocyte Stimulating Hormone Protects Pancreatic Islet Dysfunction by Peripheral Blood Mononuclear Cells in vitro / 대한이식학회지
The Journal of the Korean Society for Transplantation ; : 41-48, 2006.
Artigo em Coreano | WPRIM | ID: wpr-47494
ABSTRACT

PURPOSE:

The alpha-melanocyte-stimulating hormone (alpha-MSH) has been shown to interact with various cells of the immune and inflammatory system and down-regulate either the production or the action of the pro-inflammatory cytokines. In this study, we investigated the potential of alpha-MSH on preventing pancreatic islet cell from death and dysfunction by inflammatory cytokines released from peripheral blood mononuclear cells (PBMCs) in rat.

METHODS:

Rat pancreatic islets were co-cultured with PBMCs, stimulated by phorbol myrstic acid and ionomycin. alpha-MSH was treated to PBMCs for 2 hours before co-culture. Viability and apoptosis of islets were observed by MTT and FACS. Inflammatory cytokines and nitric oxide (NO) were measured. Insulin release from islet co-cultured with mononuclear cells was checked for the islet function.

RESULTS:

In comparison to control group, viability of islets with alpha-MSH treated mononuclear cells was increased and apoptosis was reduced significantly. Inflammatory cytokines such as TNF-alpha and IL-1beta were reduced in alpha-MSH-treated group. NO production in alpha-MSH-treated group was decreased. Insulin secretory function of islet was recovered in condition of alpha-MSH treatment.

CONCLUSION:

This study demonstrates that alpha-MSH protects cell death and preserves the secretory function of pancreatic islet cells from the pro-inflammatory reaction of mononuclear cells, and may have the potential to improve the graft survival in clinical islet transplantation.
Assuntos

Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Alfa-MSH / Ionomicina / Citocinas / Transplante das Ilhotas Pancreáticas / Ilhotas Pancreáticas / Fator de Necrose Tumoral alfa / Morte Celular / Apoptose / Técnicas de Cocultura / Sobrevivência de Enxerto Limite: Animais Idioma: Coreano Revista: The Journal of the Korean Society for Transplantation Ano de publicação: 2006 Tipo de documento: Artigo

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Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Alfa-MSH / Ionomicina / Citocinas / Transplante das Ilhotas Pancreáticas / Ilhotas Pancreáticas / Fator de Necrose Tumoral alfa / Morte Celular / Apoptose / Técnicas de Cocultura / Sobrevivência de Enxerto Limite: Animais Idioma: Coreano Revista: The Journal of the Korean Society for Transplantation Ano de publicação: 2006 Tipo de documento: Artigo