Clinical Significant of Detecting the Cytoplasm of Phospholipase A2αExpression in Patints with Chronic Obstructive Pulmonary Disease / 现代检验医学杂志

ABSTRACT

Objective To discuss the clinical significant of detecting cytoplasm phospholipase A2 alpha (cPLA2α)in patients with chronic obstructive pulmonary disease (COPD).Methods Selected treated 90 cases of patients with COPD.According to the COPD severity classification standard,they were divided into mild group of 30 cases,moderate group of 25 cases and severe group of 35 cases.In the same period of physical examination,90 cases of normal lung function healthy were control group.Used Enzyme linked immunosorbent test (ELISA)to detect cPLA2αlevel of these groups.At the same time,detected cPLA2αexpression of these groups by RT-PCR.Results cPLA2αlevel in serum of mild group was (0.039 2±0.005 1)pg/ml,gene expression was (0.68±0.01),in moderate group (0.049 8±0.007 4)pg/ml and (0.92±0.02),in severe group (0.055 4±0.008 1)and (1.35±0.02).and in Healthy controls group (0.010 2±0.006 6)pg/ml and (0.11±0.01).There were significant differences among four groups (t = 3.013 ~ 5.817,5.039 ~ 11.667,P < 0.05).Conclusion Detection of blood cPLA2αcan indicate COPD severity,and cPLA2α is the new molecular targets of diagnosis,treatment and classifica-tion COPD.