Effect of poly-L-lactic acid/amorphous calcium phosphate scaffold on the surrounding tissue calcification after implantation into the rats / 中国组织工程研究

ABSTRACT

BACKGROUND:Novel fuly biodegradable poly-L-lactic acid/amorphous calcium phosphate (PLLA/ACP) scaffold shows a good prospect of application, but whether the scaffold material has impact on the surrounding tissue calcification is unknown. OBJECTIVE: To observe the influence of PLLA/ACP scaffold material on the calcification of surrounding tissue after implantation of PLLA/ACP scaffold into rats. METHODS:A total of 48 SD rats were divided into experimental group and control group randomly. The experimental group was implanted with PLLA/ACP scaffold material, while the control group was implanted with PLLA scaffold material. At 1, 2, 4, 12 weeks after implantation, the liver function, kidney function and concentrations of calcium, phosphorus, alkaline phosphatase in serum were detected; the muscle tissue around the scaffold was colected for hematoxylin-eosin staining, Von Kossa staining, alkaline phosphatase staining and immunohistochemical staining of nuclear factor-kappa B. Then, western blot assay was used to detect the contents of interleukin-6, bone morphogenetic protein-2, and meanwhile, the contents of calcium and alkaline phosphatase in tissue homogenate were measured. RESULTS AND CONCLUSION:There was no significant difference in either group about the liver and kidney functions at each time. The content of interleukin-6 in the experimental group was less than that in the controlgroup at 2, 4 and 12 weeks after implantation (P 0.05). These findings indicate that the PLLA/ACP scaffold has a good biocompatibility and biological security, which cannot induce peripheral tissue calcification.