Effect of baicalin on BV2 cell activation damage caused by rotenone / 中国生化药物杂志

ABSTRACT

Objective To observe Effect of baicalin on BV2 cell activation damage caused by rotenone.Methods Rotenone infected BV2 cell viability were detected by MTT assay, and OX42 expression by ICC method assay, the inner cell Ca2 + content by fluorescence spectrophotometry, intracellular iron content by ICP-AES when low concentrations iron of load without damage in cells and extracellular H2 O2 and intracellular content of MDA, NO, NOS with related kits.ResuIts Rotenone exposure BV2 cell reduced cell viability, the expression of cell marker protein OX42 and intracellular Ca2 +content and intracellular iron content were all significantly increased(P<0.05), resulting in oxidative damage to cells.Baicalin reduced OX42 expression of rotenone exposure BV2 cell, increased cells survival, reduced intracellular iron content and H2 O2 release and oxidative damage of BV2(P<0.05).ConcIusion Baicalin inhibits BV2 activation and damage by rotenone-induced, and it has neuroprotective effects.The protection mechanism may relate to reduce iron into cells .