MRI tracing of stem cells:theory and application progress / 中国组织工程研究

ABSTRACT

BACKGROUND:Continuous monitoring is required in the survival, distribution, migration, proliferation and differentiation of transplanted stem cel s in vivo thereby to assess its efficacy and safety. OBJECTIVE:To analyze the characteristics of literature addressing stem cel markers and MRI tracer in the past 10 years. METHODS:The first author retrieved CNKI and Web of Science by computer for articles about MRI tracing of stem cel s published from January 2005 to December 2014, using the keywords of“stem cel s, magnetic resonance imaging, tracing technique”that appeared in the title and abstract. Then, the retrieved articles were further analyzed. RESULTS AND CONCLUSION:Currently, tracing technique has been widely used in stem cel therapy, organ transplantation, bacterial infection, gene expression and new drug development. Stem cel tracing technologies include isotope tracing, antigenic labeling, fluorescence protein labeling, fluorescent dye labeling, nuclear magnetic resonance contrast enhancer labeling, Lac-Z gene labeling, Y chromosome labeling. Recently, with the development of molecular imaging, MRI tracing techniques have been increasingly used to assess the efficacy and safety of stem cel transplantation.