Trueness Assessment for Serum Glucose Measurement Using Commercial Systems through the Preparation of Commutable Reference Materials
Annals of Laboratory Medicine
;
: 243-249, 2012.
Artigo
em Inglês
| WPRIM
| ID: wpr-47756
ABSTRACT
BACKGROUND:
Commutable reference materials (RMs) are suitable for end-users for evaluating the metrological traceability of values obtained using routine measurement systems. We assessed the performance of 6 routine measurement systems with validated secondary RMs.METHODS:
We tested the homogeneity, stability, and commutability of 5 minimally processed human serum pools according to the standard guidelines. The serum pools were assigned values as per the reference procedure of the United States Centers for Disease Control and were used to evaluate the trueness of results from 6 commercial measurement systems based on enzymaticmethods:
3 glucose oxidase (GOD) and 3 hexokinase (HK) methods.RESULTS:
The prepared RMs were validated to be sufficiently homogenous, stable, and commutable with the patient samples. Method bias varied for different systems GOD01, -0.17 to 2.88%; GOD02, 1.66 to 4.58%; GOD03, -0.17 to 3.14%; HK01, -3.48 to -0.85%; HK02, -3.83 to -0.11%, and HK03, -1.82 to -0.27%.CONCLUSIONS:
We observed that the prepared serum glucose RMs were qualified for trueness assessment. Most of the measurement systems met the minimal quality specifications.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Kit de Reagentes para Diagnóstico
/
Padrões de Referência
/
Análise Química do Sangue
/
Glicemia
/
Análise de Regressão
/
Glucose Oxidase
/
Hexoquinase
Tipo de estudo:
Estudo diagnóstico
Limite:
Humanos
Idioma:
Inglês
Revista:
Annals of Laboratory Medicine
Ano de publicação:
2012
Tipo de documento:
Artigo
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