Simultaneous Determination of Imperatorin, Isoimperatorin and Magnolin in Quchuan Capsules by HPLC / 中国药师
China Pharmacist
;
(12): 1832-1834, 2015.
Artigo
em Chinês
| WPRIM
| ID: wpr-477987
ABSTRACT
Objective:
To develop a method to simultaneously determine the content of imperatorin, isoimperatorin and magnolin in Quchuan capsules by HPLC.Methods:
A Shiseido CAPCELL PAK C18 column(150 mm × 4. 6 mm,5 μm) was used. The mobile phase consisted of acetonitrile(A)-water(B) with gradient elution at a flow rate of 1. 0 ml·min-1 and the column temperature was at 30℃. The detection wavelength was set at 278 nm from 0 to 25 min and 248 nm from 25 to 70 min. The injection volume was 10 μl.Results:
The linear range of imperatorin,isoimperatorin and magnolin was 6. 126-55. 134 μg·ml-1(r=0. 999 7), 9. 862-88. 758 μg ·ml-1(r =0.999 8) and 4.830-43.470 μg·ml-1(r =0.999 8), respectively. The average recovery was 101.5% (RSD =1. 4%), 99. 6%(RSD=1. 2%) and 100. 2% (RSD=1. 7%), respectively.Conclusion:
The method is convenient, sensitive and accurate, which can be used for the quality control of Quchuan capsules.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Idioma:
Chinês
Revista:
China Pharmacist
Ano de publicação:
2015
Tipo de documento:
Artigo
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