Preparation of Streptococcus pneumonia type 3 capsular polysaccharide conjugate vaccine / 中国免疫学杂志
Chinese Journal of Immunology
;
(12): 1361-1365, 2015.
Artigo
em Chinês
| WPRIM
| ID: wpr-478097
ABSTRACT
Objective:
To prepare Streptococcus pneumonia type 3 capsular polysaccharide conjugate vaccine.Methods:
Strep-tococcus pneumonia type 3 capsular polysaccharide was covalently linked to protein CRM197 and its immunogenicity was evaluated in infant mice.Results:
Through the preliminary research, we found that the polysaccharide was treated with 0.2 mol/L acetic acid at 85℃ for 1 h,activation grade reached to 10.0,and the radio of polysaccharide and protein was 20∶10,could induce infant mice to produce the high titers of antibodies.Conclusion:
This result shows that conjugate vaccine prepared under this condition retained intact antigenicity.It is applicable to prepare Streptococcus pneumonia type 3 capsular polysaccharide conjugate vaccine by the process condi-tions.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Idioma:
Chinês
Revista:
Chinese Journal of Immunology
Ano de publicação:
2015
Tipo de documento:
Artigo
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