Expression and significance of microRNA-100 in the tissues of pancreatic cancer / 中华消化外科杂志

ABSTRACT

Objective To investigate the effects and mechanism of expression of microRNA-100 in the tissues of pancreatic cancer and its relationship with clinicopathological factor.Methods The clinical data of 17 patients with pancreatic cancer who were admitted to the Affiliated Hospital of Guizhou Medical University between January 2013 and March 2014 were retrospectively analyzed.The surgical specimens were collected for study.The expression of microRNA-100 in the pancreatic tissues were detected by real-time fluorescent quantitative polymerase chain reaction (RT-PCR) and its relationship with clinicopathological factors was analyzed.The MIA PaCa-2 cells and CFPAC-1 cells were infected by lv-microRNA-100.MIA PaCa-2 cells were divided into the M group (microRNA-100 were infected) and N group (empty vectors were infected),and CFPAC-1 cells were divided into the C group (microRNA-100 were infected) and D group (empty vectors were infected).The expressions of microRNA-100 in the CFPAC-1 cells and MIA PaCa-2 cells were detected by RT-PCR and cell cycles were detected by flow cytometry.The expressions of Cyclin D1 protein in the CFPAC-1 cells and MIA PaCa-2 cells were detected by Western blot.Measurement data with normal distribution were presented as (x) ± s and comparison among groups were done by t test.Results The results of RT-PCR showed that the relative quantitative expression of microRNA-100 in the pancreatic cancer tissues was 0.046 ± 0.020,which was significantly different from 0.097 ± 0.017 in the adjacent tissues (t =2.789,P ＜ 0.05).There were significant differences between the tumor differentiation degree and tumor staging in patients with pancreatic cancer (t =2.563,2.135,P ＜0.05).The results of RT-PCR showed that the relative quantitative expression of microRNA-100 in the M group was 0.097 ±0.012,which was significantly higher than 0.018 ± 0.006 in the N group (t =4.410,P ＜ 0.05).The relative quantitative expression of microRNA-100 in the C group was 0.084 ± 0.021,which was significantly higher than 0.023 ± 0.010 in the D group (t =5.351,P ＜ 0.05).The results of flow cytometry showed that the percentage of cells between G0-G1 were 45.3％ ±0.7％ in the M group and 30.6％ ±0.7％ in the N group,with a significant difference (t =5.564,P ＜ 0.05).The percentage of cells between G0-G1 were 58.8％ ± 1.0％ in the C group and 42.6％ ± 0.7％ in the D group,with a significant difference (t =7.771,P ＜ 0.05).The results of Western blot showed that the relative quantitative expression of Cyclin D1 protein were 0.352 ± 0.081 in the M group and 0.872 ± 0.134 in the N group,with a significant difference (t =7.651,P ＜ 0.05).The relative quantitative expression of Cyclin D1 protein was 0.410 ± 0.121 in the C group,which was significantly different from 0.979 ± 0.232 in the D group (t =8.712,P ＜ 0.05).Conclusion Low expression of microRNA-100 in pancreatic cancer tissues may be correlated with tumor differentiation degree and tumor staging,it can inhibit tumor cells proliferation by reducing expression of Cyclin D1 protein.