Influence of Celecoxib on Radiosensization of Irradiation ̄resistant Cell Line CNE ̄2R ofNasopharyngeal Carcinoma / 医药导报
Herald of Medicine
; (12): 1439-1444, 2015.
Article
em Zh
| WPRIM
| ID: wpr-481290
Biblioteca responsável:
WPRO
ABSTRACT
Objective To investigate the effect of COX ̄2 inhibitor celecoxib on radiosensitity of irradiation ̄resistant cell line CNE ̄2R of nasopharyngeal carcinoma and the potential mechanism. Methods Via exposing to a series of X ̄ray (2, 4, 6, 8 Gy, 3 times for each dose), radio ̄resistant cell subline CNE ̄2R was established from human nasopharyngeal carcinoma cell CNE ̄2.Radiosensitivity was detected by clone formation assay.CNE ̄2R and CNE ̄2 cell lines were exposed to 25, 50, 75 μmol.L-1 celecoxib, respectively.Western blotting was used to detect the protein expression of COX ̄2.Clone formation assay was performed to measure the survival fraction of CNE ̄2 and CNE ̄2R after radiotherapy alone or radiotherapy combined with 30 μmol.L-1 celecoxib treatment.Flow cytometry was used to measure influence of radiotherapy alone or radiotherapy combined with 30 μmol.L ̄1celecoxib treatment on cell apoptosis.Number of residual γ ̄H2AX foci was observed by immunofluorescence assay. Results The colony forming assay demonstrated that the values of SF2, D0 , Dq , and N of CNE ̄2R cell subline [(0.81±0.05), (2.15±0.07) Gy, (2.94±0.08) Gy, (3.91±0.07), respectively] was significant higher than those of CNE ̄2 cell line [(0.61±0.08), (1.47±0.06) Gy, (1. 68 ± 0. 10) Gy, (3. 13 ± 0. 05), respectively]. The expression of COX ̄2 protein was significantly downregulated with increasing celecoxib concentration.Surviving fraction was decreased in both CNE ̄2 and CNE ̄2R cell lines after irradiation.After radiotherapy combined with celecoxib, apoptosis rates of CNE ̄2 and CNE ̄2R cell lines [(13.10±0.63)%, (5.30±0.75)%] were higher than those of the corresponding control groups [(4.90±0.71)%, (1.82±0.82)%].Celecoxib increased radiosensitivity in nasopharyngeal carcinoma CNE ̄2R and CNE ̄2 cell lines.The number of residual γ ̄H2AX foci after irradiation was increased by celecoxib pretreatment.The difference was statistically significant (P<0.05). Conclusion Celecoxib can enhance radiosensitivity of radio ̄resistant cell subline CNE ̄2R of human nasopharyngeal carcinoma in vitro.
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Índice:
WPRIM
Idioma:
Zh
Revista:
Herald of Medicine
Ano de publicação:
2015
Tipo de documento:
Article