Screening PCR Versus Sanger Sequencing: Detection of CALR Mutations in Patients With Thrombocytosis
Annals of Laboratory Medicine
;
: 291-299, 2016.
Artigo
em Inglês
| WPRIM
| ID: wpr-48343
ABSTRACT
BACKGROUND:
Mutations in calreticulin (CALR) have been reported to be key markers in the molecular diagnosis of myeloid proliferative neoplasms. In most previous reports, CALR mutations were analyzed by using Sanger sequencing. Here, we report a new, rapid, and convenient system for screening CALR mutations without sequencing.METHODS:
Eighty-three bone marrow samples were obtained from 81 patients with thrombocytosis. PCR primers were designed to detect wild-type CALR (product 357 bp) and CALR with type 1 (product 302 bp) and type 2 mutations (product 272 bp) in one reaction. The results were confirmed by Sanger sequencing and compared with results from fragment analysis.RESULTS:
The minimum detection limit of the screening PCR was 10 ng for type 1, 1 ng for type 2, and 0.1 ng for cases with both mutations. CALR type 1 and type 2 mutants were detected with screening PCR with a maximal analytical sensitivity of 3.2% and <0.8%, respectively. The screening PCR detected 94.1% (16/17) of mutation cases and showed concordant results with sequencing in the cases of type 1 and type 2 mutations. Sanger sequencing identified one novel mutation (c.1123_1132delinsTGC). Compared with sequencing, the screening PCR showed 94.1% sensitivity, 100.0% specificity, 100.0% positive predictive value, and 98.5% negative predictive value. Compared with fragment analysis, the screening PCR presented 88.9% sensitivity and 100.0% specificity.CONCLUSIONS:
This screening PCR is a rapid, sensitive, and cost-effective method for the detection of major CALR mutations.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Trombocitose
/
Medula Óssea
/
Análise Mutacional de DNA
/
Sequência de Bases
/
Reação em Cadeia da Polimerase
/
Seguimentos
/
Calreticulina
/
Janus Quinase 2
/
Genótipo
/
Mutação
Tipo de estudo:
Estudo diagnóstico
/
Estudo observacional
/
Estudo prognóstico
/
Fatores de risco
/
Estudo de rastreamento
Limite:
Adulto
/
Idoso
/
Feminino
/
Humanos
/
Masculino
Idioma:
Inglês
Revista:
Annals of Laboratory Medicine
Ano de publicação:
2016
Tipo de documento:
Artigo
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