Expressions and significances of micro RNA -192 and zinc finger transcription factor 1 in endometrial carci-noma / 中国基层医药

ABSTRACT

Objective To investigate the expressions and significances of micro RNA -192 and zinc finger transcription factor 1 in endometrial carcinoma.Methods 59 cases of endometrial cancer,42 cases of uterine fibroids and 42 cases of normal endometrial tissues were selected.The expressions of miR -192 and ZEB1 in different tissues were detected by using qRT -PCR.The expressions of ZEB1 proteins in difference tissues were tested by Western blot.Results The relative expressions of miR -192 in endometrial cancer tissues were (0.69 ±0.13),which were lower than the normal endometrial tissues and uterine fibroid tissues,which were (1.03 ±0.21)and (0.98 ±0.17), the differences were statistically significant (t =10.983 and 8.572,all P <0.05).The relative expressions of ZEB1 mRNA and the expressions of ZEB proteins in endometrial cancer tissues were (0.47 ±0.08)and (1.13 ±0.24), which were higher than the normal endometrial tissues and uterine fibroid tissues,which were (0.28 ±0.05),(0.89 ± 0.17),(0.31 ±0.07)and (0.87 ±0.21 ),the differences were statistically significant (t =6.834,7.053 and 5.983,6.751,all P <0.05).The expressions of miR -192 and ZEB1 in endometrial carcinoma tissues were associated with FIGO stage and lymph node metastasis (t =1.840 and 2.465,all P <0.05).Pearson correlation analysis showed that,the relative expressions of miR -192 in endometrial carcinoma tissues were negatively correlated with the expres-sions of ZEB1 (r =-0.402,P =0.002).Conclusion The expression of miR -192 in endometrial carcinoma tissues was low.It might participate in invasion and metastasis of endometrial carcinoma through acting on ZEB1.