Effect of Acupoint 830 nm Laser Irradiation on Skeletal Muscle SOD and MDA Contents in Exhaustive Exercise Rats / 上海针灸杂志

ABSTRACT

Objective To investigate the effect of acupoint 830 nm semiconductor laser irradiation on skeletal muscle SOD and MDA contents in exhaustive exercise rats.Methods Rats did exhaustive eccentric exercise by downhill treadmill running. Rats were randomized into a blank group (group A), a model group (group B), a pre-exercise semiconductor laser pre-irradiation group (group C), a post-exercise laser irradiation group (group D) and a pre- and post-exercise laser irradiation combination group (group E). Except the blank group, each of the other groups was again divided into 24 h and 48 h subgroups, and there were groups B1, B2, C1, C2, D1, D2, E1 and E2, 8 rats each. Group B rats did one exhaustive exercise by treadmill running. Group C rats received laser pre-irradiation for 7 days before they did exhaustive exercise. Group D rats received laser irradiation after the exercise. Group E rats received laser pre-irradiation for 7 days before treadmill running and combined laser irradiation after the exercise. Specimens were taken in groups B1, C1, D1 and E1 at 24 h after the exercise and in groups B2, C2, D2 and E2 at 48 h after the exercise. A 830 nm semiconductor laser transmitter was used for laser irradiation. Point Chengshan was selected in the rats. Skeletal muscle superoxide dismutase (SOD) and malondialdehyde (MDA) contents were measured in every group before and after treatment.Results There was a statistically significant difference in muscular MDA content between group B1, D1, B2 or C2 and group A (P<0.05) and between group D2 or E2 and group B2 (P<0.05). There was a statistically significant difference in muscular SOD content between group B1, C1, D1 or B2 and group A (P<0.05) and between group C2, D2 or E2 and group B2 (P<0.05).Conclusion Acupoint 830 nm semiconductor laser irradiation can restore unbalanced oxidation-reduction reactions.