Preparation of polyclonal antibody for minoxidil, development of an ELISA method for detection of minoxidil / 中国免疫学杂志
Chinese Journal of Immunology
;
(12): 1668-1673, 2015.
Artigo
em Chinês
| WPRIM
| ID: wpr-484741
ABSTRACT
Objective:
On the basis of synthetic antigens on minoxidil ,we prepared its polyclonal antibody and established a ELISA method to detect minoxidil .Methods:
We synthesized minoxidil artificial antigen with glutaraldehyde and identified by UV scan -ning.We had established a detection of minoxidil competition ELISA .Results:
The UV scan showed that the minoxidil successfully coupled to a carrier protein.The conjugate of minoxidil-BSA was used to immunize BALB/c mice.And the produced antiserum showed high titer of 112 800 in the indirect ELISA.Its ELISA curve equationy=-0.082 3 x+0.938 ( R2 =0.9811 ) , minoxidil mass concentration and the absorbance in 0.001-10 μg/ml showed a good linearity .Conclusion:
This method has been successfully preparing minoxidil artificial antigen and polyclonal antibodies and established ELISA detection method for minoxidil ,which provides a basis for the practical application of minoxidil immunoassay method .
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Tipo de estudo:
Estudo diagnóstico
Idioma:
Chinês
Revista:
Chinese Journal of Immunology
Ano de publicação:
2015
Tipo de documento:
Artigo
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