Comparison of the Anyplex II STI-7 and Seeplex STD6 ACE Detection Kits for the Detection of Sexually Transmitted Infections / 임상검사와정도관리
Journal of Laboratory Medicine and Quality Assurance
;
: 87-92, 2013.
Artigo
em Coreano
| WPRIM
| ID: wpr-48481
ABSTRACT
BACKGROUND:
Sexually transmitted infections (STI) encompass a variety of clinical syndromes caused by many pathogens that are transmitted through sexual activity. Multiplex PCR is frequently used to detect STI. In this study, two multiplex real-time PCR-based assays were used to detect STI in clinical specimens, and the concordance of the results obtained by each method was evaluated.METHODS:
A total of 626 specimens were tested using the Anyplex II STI-7 (Seegene, Korea) and Seeplex STD6 ACE Detection kits (Seegene).RESULTS:
Among the 626 individuals tested, 227 (44.2%) tested positive for STI by using Anyplex II STI-7. The prevalence rates of the various infectious microorganisms detected were as follows Chlamydia trachomatis (C. trachomatis), 19.2% (120/626); Neisseria gonorrhoeae (N. gonorrhoeae), 5.6% (35/626); Trichomonas vaginalis (T. vaginalis), 0.2% (1/626); Mycoplasma genitalium (M. genitalium), 8.1% (51/626); Mycoplasma hominis (M. hominis), 2.9% (18/626); Ureaplasma urealyticum (U. urealyticum), 17.6% (110/626); and Ureaplasma parvum, 3.7% (23/626). The concordance rates for the STI-7 and STD6 assays in detecting the various types of microorganism were as follows C. trachomatis, (99.5%); N. gonorrhoeae, (99.7%); T. vaginalis, (100%); M. genitalium, (100%); M. hominis, (100%); and U. urealyticum (99.2%).CONCLUSIONS:
A high degree of concordance was observed between the results obtained using the Anyplex II STI-7 kits and those obtained using the Seeplex STD6 ACE Detection kits.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Comportamento Sexual
/
Trichomonas vaginalis
/
Ureaplasma
/
Infecções Sexualmente Transmissíveis
/
Chlamydia trachomatis
/
Prevalência
/
Ureaplasma urealyticum
/
Mycoplasma hominis
/
Mycoplasma genitalium
/
Reação em Cadeia da Polimerase Multiplex
Tipo de estudo:
Estudo diagnóstico
/
Estudo de prevalência
Idioma:
Coreano
Revista:
Journal of Laboratory Medicine and Quality Assurance
Ano de publicação:
2013
Tipo de documento:
Artigo
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