Protective effects of Jiawei Zhenwu decoction on rats with heart failure induced by adriamycin and its mechanism / 中国生化药物杂志

ABSTRACT

Objective To explore the protective effects of Jiawei Zhenwu decoction on rats with heart failure induced by adriamycin and its mechanism.Methods The heart failure was established with adriamycin by tail intravenous injection.3 mg/kg of adriamycin was injected on 1st, 3rd and 5th week and 2 mg/kg of adriamycin was injected on 2nd, 4th and 6th week, a total of 6 times.60 rats were randomly divided into six groups, 10 rats in each group blank control group, model group, low-, middle-and high-does Jiawei Zhenwu decoction(40,20,10 g/kg, intragastric administration), and hydrochlorothiazide group(positive drug group, 10.78 mg/kg, intragastric administration).The overt behavior was observed and 24 h urine volume, Na +, K +and Cl-content in urine, cardiac function and levels of angiotensin(AngⅡ), aldosterone(ALD) and antidiuretic hormone(ADH) in plasma were compared among each group.After the rat were sacrificed, the heart was taken out and stained by HE to analyse histopathologic examination. Results The spontaneous activity and ingestion of rats improved after given Jiawei Zhenwu decoction.The 24h urine volume, Na +, K +content in urine increased of low-, middle-and high-does Jiawei Zhenwu decoction at different degrees ( P<0.05 ) , while there were no significant changes of Cl -contents; left ventricular end dilated pressure(LVEDP) decreased, left ventricular systolic pressure(LVSP), +dp/dtmax and -dp/dtmax increased in low-, middle-and high-does Jiawei Zhenwu decoction at different degrees(P<0.05);the plasma levels of angiotensin(AngⅡ), aldosterone(ALD) and antidiuretic hormone(ADH) decreased(P<0.05).The pathological examination result showed that there was significant damage of myocardial cell and infiltration of monocyte and lymphocyte, myocardial cell of high-dose group almost returned to normal and middle-and low-dose group have improvement to some extent.Conclusion Jiawei Zhenwu decoction could improve cardiac function, which may be related with the reduction of AngⅡ, ALD and ADH levels and promoting the excretion of Na +, K +so as to increase urine volume.