Dimethyl sulfoxide induces differentiation of bone marrow mesenchymal stem cells into myocardial cells / 中国组织工程研究
Chinese Journal of Tissue Engineering Research
;
(53): 26-30, 2016.
Artigo
em Chinês
| WPRIM
| ID: wpr-485717
ABSTRACT
BACKGROUND:
Numerous studies have demonstrated that bone marrow mesenchymal stem cels can be induced to differentiate into myocardial cels under certain conditions. Dimethyl sulfoxide is one of the commonly used inducers, and its mechanism is mainly by inhibiting the c-myc gene expression, thus reducing endogenous poly(adenosine diphosphate nucleotide) level.OBJECTIVE:
To study the feasibility of dimethyl sulfoxide inducing the myocardial differentiation of bone marrow mesenchymal stem cels and its optimal concentration.METHODS:
Bone marrow mesenchymal stem cels from Sprague-Dawley rats were isolated and culturedin vitro, and then induced by dimethyl sulfoxide to differentiate into myocardial cels. According to the concentrations of dimethyl sulfoxide, there were three groups 0.6%, 0.8% and 1.0% group. Additionaly, a blank control group with no induction was set up. After 72 hours of induction, induction media were removed, and cels were then cultured in normal media for 4 weeks. RESULTS ANDCONCLUSION:
Morphology and immunocytochemistry detection results confirmed that dimethyl sulfoxide could induce the differentiation of bone marrow mesenchymal stem cels into myocardial celsin vitro, and differentiated cels expressed desmin, α-actin, cTnT, cTnI and P38MAPK. The optimal induced concentration of dimethyl sulfoxide was 1.0%. Immunofluorescence double staining and electron microscope results further confirmed that dimethyl sulfoxide could induce the myocardial differentiation of bone marrow mesenchymal stem cels. Cite this articleSun LY. Dimethyl sulfoxide induces differentiation of bone marrow mesenchymal stem cels into myocardial cels. Zhongguo Zuzhi Gongcheng Yanjiu. 2016;20(1)26-30.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Idioma:
Chinês
Revista:
Chinese Journal of Tissue Engineering Research
Ano de publicação:
2016
Tipo de documento:
Artigo
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