Effects ofTangbikang on insulin like growth factor-I and its receptor expression in sciatic nerves of diabetic rats / 中国组织工程研究

ABSTRACT

BACKGROUND:Chinese medicineTangbikang can improve nerve conduction velocity in diabetic rats, and has anti-inflammatory and antioxidant effects. Insulin like growth factor-I is a key target in the treatment of diabetic peripheral neuropathy. OBJECTIVE:To observe the effect ofTangbikangon the expression of insulin-like growth factor-I and its receptor protein and mRNA in the sciatic nerve of diabetic rat model. METHODS:The experimental diabetes melitus rat models were induced by feeding high fat forage and injection with streptozotocin. After model establishment, rats were givenTangbikang 4.18, 8.35, 16.7 mg/kg per day. This study set positive control methycobal, model and normal control groups. Intragastric administration was performed for 16 weeks. RESULTS AND CONCLUSION: Compared with the model group, blood glucose levels were similar in the methycobal group, but decreased in the high-doseTangbikang group (P < 0.01). Immunohistochemical staining and real-time fluorescence quantitative PCR detection revealed that body mass, motor nerve conduction velocity, insulin like growth factor-I and its receptor protein and mRNA expressions were increased in the methycobal and high-dose Tangbikang groups (P< 0.05 orP < 0.01). Results indicated that Tangbikang can prevent and treat diabetic peripheral neuropathy by promoting insulin like growth factor-I and its receptor. Cite this articleMu XH, Sun W, Qin LL, Wu LL, Li WL, Guo X, Zhang L, Liu TH.Effects of Tangbikang on insulin like growth factor-I and its receptor expression in sciatic nerves of diabetic rats. Zhongguo Zuzhi