Simultaneous Determination of Triamcinolone Acetonide Acetate and Chloramphenicol in Chloramphenicol and Triamcinolone Acetonide Acetate Cream by HPLC-DAD / 中国药师
China Pharmacist
;
(12): 591-593, 2016.
Artigo
em Chinês
| WPRIM
| ID: wpr-485994
ABSTRACT
Objective:
To establish an HPLC method for the simultaneous determination of triamcinolone acetonide acetonide ( TAA) and chloramphenicol in the cream.Methods:
The chromatographic system consisted of a ZORBAX SB-C18 column (250 mm × 4. 6 mm, 5μm). The mobile phase consisted of methanol and phosphate buffer with gradient elution at a flow rate of 1. 0 ml·min-1, the detection wavelength was 240nm, the column temperature was ambient, and the sample size was 20 μl.Results:
The calibration curve was linear for TAA and chloramphenicol within the concentration range of 6. 12-48. 96 μg·ml-1 and 62. 1-745. 2 μg·ml-1 with the recovery of 99. 7% (RSD=1. 3%, n=9) and 99. 4%(RSD=1. 0%, n=9), respectively.Conclusion:
The method is sensitive, accurate and specific, and can be used to control the quality of chloramphenicol and triamcinolone acetonide acetate cream.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Idioma:
Chinês
Revista:
China Pharmacist
Ano de publicação:
2016
Tipo de documento:
Artigo
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