Determination of naphthopyrone glycosides and anthraquinone aglycone in Cassia tora L by quantitative analysis of multi-components by single marker / 中国生化药物杂志

ABSTRACT

Objective To develop a method of quantitative analysis of multi-components by single marker(QAMS) for determination of effective components of naphthopyrone glycosides and anthraquinone aglycone in Cassia tora L.Methods The separation was performed on a Sunfire C18 column (4.6 mm ×250 mm,5 μm), and column temperature 30 ℃.Aetonitrile-0.1% H3PO4 was used as the mobile phase with gradient elution.The detection wavelength was at 278 nm and 284 nm.Relative correction factor by determination of Between aurantio-obtusin and rubrofusarin-gentiobioside or chrysophanol or physcion.the method was evaluated for reproducibility, and the difference between calculated and measured values was compared. Results Rubrofusarin-gentiobioside, aurantio-obtusin, chrysophanol and physcion respectively 0.0503-1.5078, 0.3197-9.5899, 0.5070-15.2108, 0.4027-12.0814μg showed a good linear relationship, and the regression equation is Y =4.95X +2.01(R2 =0.9998),Y =1.03X +0.03(R2 =0.9999),Y=3.98X-0.12(R2 =0.9993),Y =4.81X +0.26(R2 =0.9996).The quantitative results of six batches of Cassia tora L by QAMS was basically consistent with that by external standard method.Conclusion The QAMS method was reliable and accurate, which might be used for the quality control of Cassia tora L.