Protective effect of extract from Shuilu Erxiandan on oxidative injury of structure and function of human sperm membrane / 国际检验医学杂志

ABSTRACT

Objective To explore the mechanism of Shuilu erxiandan against male′s infertility and asthenoospermia .Methods Human sperm with normal physiological function were collected ,and then sperm suspension were divided into the normal group ,the model group ,Vitamin C group and the groups of large ,medium and small dose of Shuilu erxiandan .The reactive of species was made from hypoxanthine-xanthine oxidase .In the oxygen environment ,Vitamin C(0 .25 g/L) and different content (1 .0 ,0 .5 ,0 .25 g/mL) of extract from Shuilu erxiandan were hatched with sperm .Then the function of sperm membrane in every group was evalu-ated using hypoosmoticswelling .Meanwhile the content of MDA and SOD were detected .Results Expansion of sperm and sperm motility in all the groups of vitamin C and large ,medium and small dose of Shuilu erxiandan′s extract were significantly higher than that in the model group at time point of 1 ,3 and 6 h (P< 0 .05) .Also ,medium dose of Shuilu erxiandan (0 .5 g/mL) displayed more effective on expansion of sperm and sperm motility compared with that in the vitamin group C (P< 0 .05) .All the groups of vitamin C and large ,medium and small dose of Shuilu erxiandan′s extract significantly indicated descreased MDA content and improved SOD activity compared with that in the model group (P< 0 .05) .Also ,this effect of medium dose of Shuilu erxiandan (0 .5 g/mL ) on MDA and SOD activity were superior to that in vitamin C group ,which showed significant difference (P< 0 .05) .Conclusion Ex-tract from Shuilu erxiandan could significantly protect structure and function of sperm membrane ,which is correlated with interve-ning its lipin peroxidation .