Protective effects of astragalosides on pancreatic tissue in diabeticrats / 国际中医中药杂志

ABSTRACT

Objective To investigate the protective effects of astragalosides on pancreatic tissue in diabetic rats.Methods The experimental diabetic rat models were made by feeding with high fat and sugar and injecting STZ, and according to blood glucose level, the rat models were randomly devided into five groups a diabetic model control group, an astragalosides low (30 mg/kg), a medium (60 mg/kg), a high (120 mg/kg) dose treated groups and a metformin hydrochloride 200 mg/kg treated group (n=16), and selected another 16 same-aged rats as a normal control group. 72 h after the models were made, the drugs were given by intragastric administration for 4 weeks, once a day. Before and after the drugs were given 7, 14, 21, 28 days, the level of plasma glucose (FPG) and insulin (FINS) were determined, and the ISI and HOMA-IR were calculated, the level of T-AOC and the content of ROS in serum were detected, the activity of SOD, CAT and the content of MDA in pancreatic tissue were detected, the histopathological changes of pancreatic tissue and pancreatic cells apoptosis were observed, and the apoptosis index were analyzed.Results Four weeks later, compared with the diabetic model control group, the FINS (9.34 ± 1.67 mIU/ml, 10.46 ± 1.51 mIU/mlvs.7.37 ± 1.39 mIU/ml) and the level of ISI (6.82 ± 0.96, 6.52 ± 0.79vs.7.35 ± 0.84) in astragalosides medium and high dose treated groups were significantly increased (P<0.05 orP<0.01), the level of HOMA-IR (43.62 ± 5.26, 29.37 ± 4.51vs.61.70 ± 5.85) were significantly decreased (P<0.05 orP<0.01). The level of FPG (9.32 ± 2.57 mmol/Lvs.17.15 ± 2.54 mmol/L) in astragalosides high dose treated group was significantly decreased (P<0.01), the level of T-AOC (12.20 ± 3.36 U/L vs.9.34 ± 2.59 U/L) in serum was significantly increased (P<0.01). The activity of SOD (11.52 ± 2.31 U/mg, 15.29 ± 2.50 U/mgvs.7.53 ± 1.86 U/mg) in serum of astragalosides medium and high dose treated groups were significantly increased (P<0.05 orP<0.01). The content of MDA (2.83 ± 0.37 nmol/mg, 1.92 ± 0.31 nmol/mgvs. 4.08 ± 0.42 nmol/mg) in pancreatic tissue of astragalosides medium, high dose treated groups were significantly decreased (P<0.05 orP<0.01); the activity of SOD (11.52 ± 2.31 U/mg, 15.29 ± 2.50 U/mgvs.7.53 ± 1.86 U/mg) and CAT (392.28 ± 38.05 U/g, 461.73 ± 42.52 U/gvs. 280.46 ± 29.61 U/g) were significantly increased (P<0.05 orP<0.01).Conclusion Astragalosides had protective effects on pancreatic tissue in diabetic rats.