Simultaneous Determination of 5 Active Ingredients in Begonia Grandis Dry from Different Habitats by UP-LC / 中国药师
China Pharmacist
;
(12): 780-782, 2016.
Artigo
em Chinês
| WPRIM
| ID: wpr-490870
ABSTRACT
Objective:
To establish a method for the determination of protocatechuic acid, protocatechuic, catechin, epicatechin and rutin in Begonia grandis Dry.Methods:
The 5 active ingredients in the samples were extracted by 70% methanol and analyzed by ultra performance liquid chromatography (UPLC). Chromatographic separation was performed on a Waters ACQUITY HSS T3(2. 1 mm × 100 mm, 1. 8 μm) column with gradient elution, and the mobile phase was solvent A acetonitrile and solvent B0. 2% glacial acetic acid (0-12 minA5→25 and B95→75;12-30 minA25→50 and B75→45). The effluent was monitored by a VWD detector set at 280 nm. The flow rate was set at 0. 2ml/min and the injection volume was 1μl. The column temperature was maintained at 30℃.Results:
The calibration curves were all linear (r≥0. 99) over the test ranges. The average recovery was 96. 92%-99. 75%(n=6).Conclusion:
The established method can rapidly obtain an accurate and reproducible result, which can be used to control the quality of Begonia grandis Dry.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Idioma:
Chinês
Revista:
China Pharmacist
Ano de publicação:
2016
Tipo de documento:
Artigo
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