Study on Preparation of Immobilized Thrombin Treated with Silk Fibroin / 中国药师
China Pharmacist
;
(12): 671-674, 2016.
Artigo
em Chinês
| WPRIM
| ID: wpr-490895
ABSTRACT
Objective:
To investigate the effects of silk fibroin on the immobilization of thrombin.Methods:
The immobilized thrombin was prepared using silk fibroin as the carrier and glutaraldehyde as the crosslinking agent. With activity yield as the index, the process conditions of silk fibroin immobilized thrombin were determined by an orthogonal test.Results:
The optimum process con-ditions of immobilized thrombin treated with silk fibroin were as followsthe immobilization time was 6 h, the enzyme dosage was 2 400 NIH·g-1 casein, the temperature was 25℃ and pH was 7. 6. The activity recovery of immobilized thrombin was 67. 22%. Conclu-sionSilk fibroin has the positive immobilization effect on thrombin.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Idioma:
Chinês
Revista:
China Pharmacist
Ano de publicação:
2016
Tipo de documento:
Artigo
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