The renal ischemia reperfusion injury caused by carbon dioxide pneumoperitoneum and the protective effect of propofol-induced nuclear factor E2-related factor 2 expression in rats / 中国小儿急救医学

ABSTRACT

Forty-eight male Wistar rats,weighing 280 to 320 g,were selected and prepared by fasting but free drinking for 12 hours before the experiment.They were randomly divided into four groups by random number table (12 rats in each group)control group,ischemic group,reperfusion group and propofol group.Control groupnormal saline infusion[10 ml /(kg?h)]for 1 h in caudal vein without any other treatment.Ischemic groupon the basis of control group,supply with 20 mmHg(1 mmHg =0.133 kPa)pneumoperitoneum pressure for 1 h. Reperfusion groupon the basis of ischemic group,keep pneumoperitoneum pressure open for 30 min.Propo-fol groupinfusion propofol in caudal vein [10 ml /(kg?h),saline 10-fold dilution],given 20 mmHg pneu-moperitoneum pressure for 1 h,then kept this pressure open for 30 min.The expression changes of blood urea nitrogen(BUN),creatinine(Cr),superoxide dismutase(SOD),malondialdehyde(MDA)and nuclear factor E2-related factor 2(Nrf2)were detected individually.Results The levels of BUN,Cr and MDA gradually increased,while the level of SOD decreased in control group,ischemic group and reperfusion group (P ﹤0.05,respectively).Compared with ischemic group and reperfusion group,the levels of BUN,Cr and MDA decreased significantly,and the level of SOD increased markedly in propofol group(P ﹤0.05,respectively). The expression of Nrf2 protein and mRNA gradually increased in the control group,ischemia group,reperfu-sion group and propofol group (P ﹤0.05).Conclusion Carbon dioxide pneumoperitoneum can cause renal IRI in rats.Propofol has a protective effect of IRI by regulating the expression of Nrf2 protein.