Experimental study on inhibitory effect ofβ-elemene on ECV-304 cells'proliferation / 中国医师杂志
Journal of Chinese Physician
; (12): 5-8, 2015.
Article
em Zh
| WPRIM
| ID: wpr-491905
Biblioteca responsável:
WPRO
ABSTRACT
Objective To investigate the growth inhibition of β-elemene in ECV-304 cells,and study it's meaning to anti-angiogenesis in tumors.Methods The IC50 value of β-elemene in ECV-304 cells were got through MTT assay.Under treatment of β-elemene in ECV-304 cell,flow cytometry(FCM)was used to investigated the changing of cell cycle,electron microscope was used to show the morphological al-terations,agarose gel electrophoresis was used to detect effection on cells'DNA.Results The proliferation of ECV-304 cells was significantly inhibited by β-elemene,the IC50 value was (58.3 ±8.1)μg/ml.By FCM,we observed Cell cycle was significantly arrested in G0 /G1 phase under 60μg/ml treatment(P <0.05),apoptotic cells increased with the increase of drug concentrations.Typical apoptotic morphological alterations were found under electron microscope.Characteristic DNA-ladder standing for apoptosis was showed in agarose gel electrophoresis.Conclusions β-elemene can significantly inhibit ECV-304 cells'pro-liferation By inducing apoptosis.
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WPRIM
Idioma:
Zh
Revista:
Journal of Chinese Physician
Ano de publicação:
2015
Tipo de documento:
Article