Inhibitory effect of Bletilla striata extracts on bleomycin-induced pulmonary fibrosis in rats / 国际药学研究杂志

ABSTRACT

Objective To investigate the inhibitory effect of Bletilla striata extracts(BSE)on bleomycin-induced pulmonary fibrosis(PF)in rats and study the chemical characteristics of the active component. Methods B. striata was extracted with 95%ethyl?alcohol to obtain the ethanol extract. The residue was extracted with water and precipitated with ethanol to obtain the B. striata polysac?charide(BSP). Sprague Dawley male rats were intratracheally administered of bleomycin(BLM,5 mg/kg)to induce PF. PF Rats were administered with ethanol BSE(300 mg/kg body mass)and BSP(300 mg/kg body mass)once daily for 28 d by gavage. On 14 d and 28 d, the lungs were harvested to observe the pathological and collagen deposition changes. The lung coefficient and the content of hydroxy?proline(HYP)were also determined. The phenol-sulfuric acid method was used to detect the total sugar content,the relative molecular mass was detected by high performance gel permeation chromatography(HPGPC)and the monosaccharide components were analyzed by high performance liquid chromatography(HPLC). Results Compared with PF model group,the histopathological changes in lung tissue treated with BSP group were obviously improved ,the level of HYP in lung tissues and the lung coefficient were significantly decreased (P<0.05,P<0.01). But the group treated with ethanol BSE had no significant improvement. The content of BSP was 81.41%,and the number average molecular weight(Mn)of BSP was about 2.23 × 105. The BSP was mainly composed of glucose and mannose. Conclusion HYP could significantly decrease lung HYB and collagen deposition of PF rats,and has the potiential for inhi?bition of PF.