Effect of Resveratrol on the Expression of MCP-1 and BNP in Myocardial Cells of Rats with Acute Pulmonary Embolism / 华中科技大学学报(医学版)

ABSTRACT

Objective To examine the effect of resveratrol on the expression of monocyte chemoattractant protein-1(MCP-1)and brain natriuretic peptide(BNP)in myocardial cells of rats with acute pulmonary embolism.Methods Ninety-six SD rats were randomly divided into 2 groupsthromboembolism group (group T)and resveratrol + thromboembolism group (group TR),each group having 3 subgroups(n=1 6 each)in terms of different time points(1 h,4 h,8 h).The model of acute pulmonary embolism was established by infusion of autologous thrombi via the catheter inserted from the right jugular vein to the pulmo-nary artery.In group TR,resveratrol at 10 mg/kg (dissolved in 1%DMSO solution)was injected to the rats via the tail vein.Animals were sacrificed at 1,4 and 8 h after the model establishment.The rat hearts were harvested,fixed in 10%paraformaldehyde and paraffin-embedded.The expression levels of MCP-1 and BNP were detected by immunohistochemistry (SP method).Results Both MCP-1 and BNP were expressed in myocardial cells in group T and TR and gradually increased with time.The expression levels of MCP-1 and BNP were much lower in group TR than in group T at 4 and 8 h,with difference being statistically significant(P<0.05).Conclusion The expression of MCP-1 and BNP tended to increase with time in both groups.Resveratrol may protect the heart tissues by suppressing the expression of MCP-1 in myocardial tissues of rats with a-cute pulmonary embolism.