The kinetics of interaction between Fructose 1,6 - bisphosphat aldolase and derivatives of heparin

ABSTRACT

Fructose 1,6 bisphosphate adolase A and C (FPA A4 and C44) have been found to bind specifically to heparin in physiological ionic strength. The researcher found that activity of FPA was inhibited by heparin. The inhibition of FPA A4 and C4 depend on the degree of sulfation. Inhibition activity FPA of heparin increase directly proportional to molecule lenghth. Kinetic studies showed that inhibitory mode of heparin on FPA was the linear mixed type inhibition, in which velocity can be driven to zero at high heparin concentration