Application of flow cytometry in the judgment of apoptosis and prognosis in patients with acute leukemia / 中国基层医药

ABSTRACT

Objective To investigate the clinical significance of flow cytometry(FCM)in cell apoptosis and prognosis in patients with acute leukemia.Methods FCMpropidium iodide(PI)DNA quantitative stain was used in 55 patients with acute leukemia(acute lymphoblastic leukemia treatment in 20 cases,alleviating period of 25 cases, 10 cases of acute non lymphocytic leukemia)to detect cell apoptosis and DNA cycle content changes,and compared with the control group.Results Acute leukemia cell apoptosis percentage Apo% in remission period was the highest, and it was (26.70 ±4.11)%,which of the initial treatment group was (10.23 ±1.05)%,remission with early treat-ment group and control group,the differences were statistically significant(t =3.970,2.341,all P <0.05).Leukemia DNA index (DI),aneuploid (AN rate),S phase cell percentage (SPF)value were (2.03 ±0.66 ),40% and (15.68 ±2.17)respectively,which were significantly increased.Conclusion The content of DNA in patients with acute leukemia is abnormal,the amount of apoptosis,SPF and other indicators have certain reference value for the prognosis of patients with acute leukemia.