HPLC Fingerprint and LC-TOF-MS Analysis on Extract from Roots of Gentiana macrophylla / 中草药·英文版
Chinese Herbal Medicines
; (4): 245-251, 2012.
Article
em Zh
| WPRIM
| ID: wpr-499734
Biblioteca responsável:
WPRO
ABSTRACT
Objective Establishing a fingerprint method to identify the characteristic chemicals in the roots of Gentiana macrophylla and evaluate their quality.Methods RP-HPLC was developed for fingerprint analysis and determination of four ingredients in G macrophylla roots from different sources.LC-ESI-TOF-MS was employed to identify the chromatographic peaks of the fingerprint.Results Five common peaks were identified by comparing their retention time with reference secoiridoid glucosides.Eight major peaks in chromatographic fingerprint were analyzed by on-line LC-ESI-TOF-MS.Four secoiridoid glucosides were identified based on their MS data.Conclusion The method is specific and could be served for the quality identification and comprehensive evaluation of G macrophylla.
Texto completo:
1
Índice:
WPRIM
Tipo de estudo:
Prognostic_studies
Idioma:
Zh
Revista:
Chinese Herbal Medicines
Ano de publicação:
2012
Tipo de documento:
Article