Isolation and Purification of Isoaloeresin D and Aloin from Aloe vera by High-speed Counter-current Chromatography / 中草药·英文版
Chinese Herbal Medicines
; (4): 148-152, 2010.
Article
em Zh
| WPRIM
| ID: wpr-499821
Biblioteca responsável:
WPRO
ABSTRACT
Objective To develop an efficient method to isolate and purify the main components isoaloeresin D and aloin from Aloe vera for its industrial production. Methods High-speed counter-current chromatography was used to isolate isoaloeresin D and aloin in a one-step separation from dried crude extract ofA. vera. The biphasic solvent system the lipophilic phase was selected as the mobile phase and the apparatus was rotated at 840 r/min. The effluent was detected at 254 nm. Results Isoaloeresin D (53.1 mg) and aloin (106.9 mg) were separated from the crude extract (384.7 mg) with the purities of 98.6% and 99.5%, respectively. Conclusion HSCCC is a powerful technique for isolation and separation of chemical composition from aloe.
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Índice:
WPRIM
Idioma:
Zh
Revista:
Chinese Herbal Medicines
Ano de publicação:
2010
Tipo de documento:
Article