A method for microbial decontamination ofAcanthamoeba cultures using the peritoneal cavity of mice
Asian Pacific Journal of Tropical Biomedicine
;
(12): 763-766, 2015.
Artigo
em Chinês
| WPRIM
| ID: wpr-500474
ABSTRACT
Objective:
To evaluate whether the inoculation of contaminated cultures in the peritoneal cavity of mice could implement decontamination ofAcanthamoeba cultures.Methods:
Suspensions ofAcanthamoeba,Acanthamoeba polyphagaATCC30461, or Acanthamoeba spp. isolated from soil (UnB13 strain) were inoculated in the peritoneal cavity of Swiss mice (n = 24). After 1, 6, 12, or 24 h of exposure the peritoneal cavity was washed and assessed for the presence of bacteria, fungi, andAcanthamoeba.Results:
After 1 h of intraperitoneal inoculation at least 97% of the bacteria and 96% of the fungi (PConclusions:
Our data demonstrated that this technique has great potential for decontamination ofAcanthamoeba cultures in a short period of time.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Idioma:
Chinês
Revista:
Asian Pacific Journal of Tropical Biomedicine
Ano de publicação:
2015
Tipo de documento:
Artigo
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